Rat Visfatin ELISA Kit

Assay Genie offers a sandwich ELISA, double antibody kit designed for the quantitative measurement of visfatin (also known as nicotinamide phosphoribosyltransferase, or NAMPT) in rat samples. The assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and uses a colorimetric detection method. Visfatin is described as a multifunctional protein involved in regulating energy metabolism, inflammation, and insulin sensitivity, with elevated levels linked to metabolic disorders such as obesity, diabetes, and cardiovascular diseases.

Reported linearity at 1:2, 1:4, and 1:8 dilutions was 92-100%, 92-104%, and 90-105% for serum; 82-98%, 82-97%, and 88-101% for EDTA plasma; and 80-93%, 82-96%, and 81-100% for heparin plasma. Recovery ranges were 85-103% (average 95%) for serum, 92-104% (average 96%) for EDTA plasma, and 94-102% (average 98%) for heparin plasma.

The procedure involves equilibrating reagents and TMB substrate to room temperature, loading standards, samples and blanks onto the pre-coated plate, incubating at 37°C for 90 minutes, adding a biotin-labelled detection antibody with a 60-minute incubation, binding HRP-Streptavidin for 30 minutes, developing colour with TMB substrate in the dark for 10-20 minutes, and reading absorbance at 450 nm after adding stop solution. The kit is stored at 2-8°C for 12 months.