Rat VIP/Vasoactive intestinal peptide ELISA Kit

From Assay Genie, this immunoassay is designed for the precise measurement of Vasoactive Intestinal Peptide (VIP) levels in rat samples. Using a Sandwich ELISA, Double Antibody format with a colorimetric detection method, the kit offers a sensitivity of 4.688 pg/mL and a range of 7.813-500 pg/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reagents to be stored at 2-8°C for 12 months.

VIP is a neuropeptide with multiple physiological functions, including regulating smooth muscle relaxation, immune responses, and neurotransmitter release. Dysregulation of VIP has been associated with inflammatory bowel disease, asthma, and neurological disorders, highlighting its importance as a biomarker for research applications. The associated Uniprot ID is P01283, with synonyms including VIP, vasoactive intestinal peptide, VIP peptides, and PHM27.

Linearity was demonstrated in serum (85-105%), EDTA plasma (83-101%), and heparin plasma (81-100%) across 1:2, 1:4, and 1:8 dilutions. Recovery ranges from 85-103% (average 97%) in serum, 85-100% (average 91%) in EDTA plasma, and 89-101% (average 92%) in heparin plasma.