Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

VGF is a 68 kDa neuropeptide precursor expressed in neurons and neuroendocrine tissues. Post-translational processing of the precursor liberates bioactive peptides such as TLQP-21, TLQP-62, AQEE-30, and NERP-1, which participate in energy homeostasis, synaptic plasticity, hippocampal neurogenesis, and pain modulation.

This quantitative immunoassay from Assay Genie supports research into metabolic disorders, depression-like behaviour, Alzheimer's-related neurodegeneration, and neuropathic pain pathways, where endogenous VGF levels serve as a biomarker of neuronal and neuroendocrine activity.

The assay uses a pre-coated microplate with anti-VGF capture antibody, a biotinylated detection antibody, and streptavidin-HRP chemistry, providing reagents for a single plate run.

For reagent stability, store kit components refrigerated, protect the streptavidin-HRP from light, and limit repeated freeze-thaw cycles of prepared standards. Common applications include quantifying VGF in hypothalamic and hippocampal tissue homogenates, sciatic nerve injury models, primary neuronal cell culture supernatants, and conditioned media from neuroendocrine cell lines.