Rat VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit (RTES01205)

A colorimetric sandwich ELISA from Assay Genie measures VEGF-A in rat serum, plasma and other biological fluids, including cell culture supernatants. The kit is described as high quality with a simple protocol for measuring VEGF-A in serum, plasma and lysate samples. The specified assay time is “3 h 30 min” and the stated sample volume is “100 μL”.

Stated performance specifications include a sensitivity of “18.75 pg/mL” and a detection range of “31.25-2000 pg/mL”, with recovery of “80%-120%”. Recovery testing reported an average recovery of “95” for EDTA plasma and “95” for cell culture media, with ranges of “90-102” and “89-101” respectively. Reported intra-assay CV values are “4.2%”, “4.58%” and “4.05%”, while inter-assay CV values are “8.05%”, “8.04%” and “8.06%”. The kit recognizes rat VEGF-A, and no significant cross-reactivity or interference between rat VEGF-A and analogues was observed.

Supplied components include a dismountable micro ELISA plate, reference standard and sample diluent, concentrated biotinylated detection antibody, concentrated HRP conjugate, detection antibody and HRP conjugate diluents, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Storage is specified at “2-8℃”.