Rat VEGF-A PharmaGenie ELISA Kit (SBRS1707)

The Rat VEGF-A PharmaGenie ELISA Kit from Assay Genie provides quantitative measurement of vascular endothelial growth factor A, a homodimeric signaling protein that drives angiogenesis and vascular permeability. The assay is calibrated for rat VEGF-A with specificity confirmed against the rat 164 amino acid VEGF-A isoform and minimal cross-reactivity toward mouse or human VEGF homologs. Researchers studying tumor vascularization, ischemic tissue repair, or inflammatory responses rely on accurate VEGF-A quantification in rat preclinical models. The microplate format supports parallel processing of samples across a single plate, and the procedure accommodates cell culture supernatants, cell lysates, plasma, serum, and tissue lysates. Components include pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant VEGF-A standards, wash buffer, and substrate solution. The recommended assay workflow involves sample incubation, washing steps, and color development read on a standard microplate reader. Unopened kits should be stored refrigerated at 2-8 degrees C, and reconstituted reagents remain stable through limited freeze-thaw cycles as outlined in the lot-specific certificate of analysis.