Rat VDR/Vitamin D Receptor ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody method to quantify Vitamin D Receptor (VDR) levels in rat-derived samples. It is intended for research applications investigating Vitamin D biology, including its roles in calcium homeostasis, immune function, and cell proliferation, as well as conditions linked to dysregulated Vitamin D receptor signalling such as osteoporosis, autoimmune disorders, and certain cancers.

The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is stored at 2-8°C for 12 months. Reported sensitivity is 0.188 ng/mL with a working range of 0.313-20 ng/mL. Linearity across 1:2, 1:4 and 1:8 dilutions is 90-103% / 91-104% / 85-100% for serum, 87-94% / 83-97% / 85-97% for EDTA plasma, and 84-99% / 83-99% / 85-94% for heparin plasma. Recovery averages 96% in serum, 93% in EDTA plasma, and 99% in heparin plasma.

The protocol follows a six-step procedure: reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance reading at 450 nm after addition of stop solution. The kit is classified within the Epigenetics and Nuclear Signaling research area, with the target also referred to as Vdr or NR1I1 (Nuclear receptor subfamily 1 group I member 1).