Rat V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) ELISA Kit

Quantify c-Jun, the nuclear transcription factor encoded by the JUN oncogene and a core subunit of the AP-1 complex, with this rat-reactive immunoassay from Assay Genie. The kit is configured for research use only and quantifies endogenous JUN protein in tissue homogenates and cell lysates prepared from rat sources, supporting studies of immediate-early gene activation, cellular stress signaling, proliferation control, and apoptosis regulation.

Reagents are supplied in a 96-assay configuration, providing capacity for standard curves and replicate samples across multiple experimental conditions. Typical workflows pair this readout with western blot confirmation of JUN phosphorylation status or with PCR profiling of downstream AP-1 target genes, and the assay is broadly applicable to neuronal, hepatic, and oncological research models where rat c-Jun dynamics are investigated.

Reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles, while the plate and detection reagents equilibrate to room temperature before each run. Protect light-sensitive components from exposure during assay setup. Detailed standard curve preparation, dilution guidelines, and protocol notes are provided in the package insert to ensure reproducible quantitation between experiments and between operators.