Rat uPAR ELISA Kit

From Assay Genie, the Rat uPAR ELISA Kit is a Sandwich ELISA, Double Antibody assay designed for the quantitative measurement of Urokinase Plasminogen Activator Receptor (uPAR) in rat samples. The kit is reactive with rat and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is performed colorimetrically on a pre-coated microplate, with a TMB substrate and absorbance read at 450 nm. Target synonyms include PLAUR, uPAR, and Urokinase plasminogen activator surface receptor, and the associated identifiers are UniProt P49616, KEGG K03985, and Gene ID 50692.

Reported performance characteristics include a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL. Linearity has been demonstrated at dilution ratios of 1:2, 1:4, and 1:8 in serum (n = 5) at 98-104%, 85-103%, and 86-103%; in EDTA plasma (n = 5) at 84-100%, 83-94%, and 85-96%; and in heparin plasma (n = 5) at 80-95%, 81-99%, and 88-95%. Recovery ranges are 85-102% (average 95%) for serum (n = 5), 85-98% (average 92%) for EDTA plasma (n = 5), and 88-101% (average 96%) for heparin plasma (n = 5). The kit should be stored at 2-8°C for 12 months, with shipping also at +4°C.

UPAR is described as a key receptor involved in the regulation of urokinase plasminogen activator, playing a role in cell migration, tissue remodeling, and cancer progression. The kit is positioned for research applications including the study of cancer, cardiovascular diseases, and other conditions where accurate measurement of UPAR levels can support investigation of disease mechanisms and potential therapeutic targets.