Rat Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit

UCP1 is a mitochondrial inner-membrane carrier protein that drives non-shivering thermogenesis in brown adipose tissue. This quantitative immunoassay from Assay Genie enables measurement of rat UCP1 in sample matrices including tissue homogenates, cell lysates, and cell culture supernatants. The plate-based format supports 96 assays per run, fitting routine screening of thermogenic responses in adipocyte models, mitochondrial bioenergetics studies, and research on obesity, type 2 diabetes, and related metabolic disorders. Each kit includes pre-coated wells, rat UCP1 standards, biotinylated detection antibody, streptavidin-HRP conjugate, substrate solution, and a complete protocol, with shelf-life and storage guidance provided in the manual. The workflow follows a plate-immobilized antibody binding sequence with an absorbance readout, compatible with laboratories running moderate-throughput brown adipose tissue activation studies or browning-white-adipocyte investigations. Stated compatibility extends to clarified lysates from differentiated adipocyte cultures, which are commonly used in vitro to monitor UCP1 induction after pharmacological or genetic manipulation.