Rat UCHL1 ELISA Kit

Assay Genie's Sandwich ELISA (Double Antibody) kit enables precise measurement of UCHL1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) levels in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. UCHL1 is a key enzyme involved in protein degradation and regulation, playing a crucial role in maintaining cellular homeostasis and implicated in neurodegenerative diseases such as Parkinson's and Alzheimer's. The kit uses a colorimetric detection method with a sensitivity of 37.5 pg/mL and a range of 62.5–4000 pg/mL, with storage recommended at 2-8°C for 12 months.

Linearity validation showed for serum (n=5): 88-102% at 1:2, 90-103% at 1:4, and 88-104% at 1:8 dilutions. EDTA plasma (n=5) yielded 86-98%, 84-94%, and 87-99% across the same dilutions, while heparin plasma (n=5) yielded 80-96%, 81-93%, and 84-99%. Recovery data: serum (n=5) 85-105% with 95% average, EDTA plasma (n=5) 90-99% with 93% average, and heparin plasma (n=5) 92-103% with 98% average.

The assay procedure involves reagent equilibration, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after adding stop solution. The kit falls within the Signal Transduction research area and is associated with UniProt ID Q00981.