Rat TYR (Tyrosinase) ELISA Kit (RTES00871)

Tyrosinase is the rate-limiting copper-containing oxidase that catalyzes the first two steps of melanogenesis, converting tyrosine to L-DOPA and then to dopaquinone. Researchers studying pigmentation biology, melanoma progression, vitiligo, and ocular melanocyte function rely on quantitative measurement of TYR levels to track enzyme expression across experimental conditions, including drug treatments, genetic knockouts, and disease-model comparisons in rodents.

This colorimetric immunoassay from Assay Genie is configured to quantify tyrosinase in rat-derived specimens, supporting studies in rodent pigmentation models, dermatology, and translational melanoma research. Each kit provides reagents for 96 individual determinations run on a standard microplate, with all components calibrated for use with rat serum samples and matched plasma specimens.

The assay uses a matched antibody pair specific to rat TYR, enabling quantitative detection of the target protein through a sequential binding format. Reconstituted standards and diluted samples are loaded directly into pre-coated wells, and bound enzyme is revealed through a chromogenic substrate reaction read on a standard plate reader. Stop solution terminates color development and the resulting absorbance is proportional to TYR concentration in the original specimen.

Unopened kits should be stored refrigerated and protected from light; reconstituted working reagents should be kept cold and used promptly to avoid loss of activity from repeated freeze-thaw cycling. The complete package includes a pre-coated plate, detection antibody, lyophilized standard, chromogenic substrates, wash buffer, and protocol insert sufficient for the full 96-assay workflow.