Rat Tumor Necrosis Factor Beta (TNFb) ELISA Kit

This Assay Genie sandwich ELISA kit quantifies rat TNF-beta (also known as lymphotoxin-alpha, LT-α), a cytokine produced primarily by lymphocytes that plays a central role in inflammatory signalling, lymphoid organ development, and autoimmune disease models. The assay uses a pre-coated 96-well microplate format and produces a measurable absorbance signal after approximately 3.5 hours of incubation.

Reported performance characteristics include a detection range of 15.6 to 1000 pg/mL and a sensitivity of less than 10 pg/mL, with a sample volume of 50 uL per well. The kit is intended for research use only and is commonly applied to studies of rheumatoid arthritis, inflammatory bowel disease, and transplant rejection in rodent models.

Reconstituted standards and biotinylated detection antibody should be stored at -20 °C, while the sealed microplate and remaining components are kept refrigerated. Allow all reagents to reach room temperature before use and avoid repeated freeze-thaw cycles of working solutions to preserve antibody binding activity.