Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

Assay Genie's quantitative immunoassay kit enables researchers to measure Tryptophan Hydroxylase 2 (TPH2), the brain-enriched isoform that catalyzes serotonin biosynthesis in raphe nuclei and other serotonergic neurons. Each kit supplies sufficient reagents for 96 assays and uses a colorimetric readout compatible with standard microplate readers.

Intended for in vitro research use only, the assay accepts tissue homogenates and cell lysates from rat sources, supporting investigations into central nervous system serotonin metabolism, mood disorder models, and antidepressant pharmacology. The microplate format streamlines parallel processing of multiple experimental samples, and rat-specific reactivity makes the kit well suited to rodent neuroscience workflows.

Stability notes: keep components refrigerated between uses and protect from light. Each kit provides pre-coated plate wells, detection antibody, calibrator standards, and chromogenic substrate. The antibody pair targets the neuronal TPH2 isoform with limited recognition of peripheral TPH1, supporting clean quantification in brain tissue, midbrain preparations, and cultured neuronal cell lysates.