Rat Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

Built for quantitative measurement of tryptophan hydroxylase 1 in rat-derived samples, this Assay Genie ELISA Kit targets the peripheral serotonin-biosynthesis enzyme encoded by the Tph1 gene, the isoform expressed outside the central nervous system in enterochromaffin cells, mast cells, and the pineal gland. Researchers studying gut-derived serotonin signaling, mucosal immune responses, pulmonary vascular remodeling, or mast cell biology use the kit to quantify TPH1 protein across tissue homogenates and cell lysates in an in vitro research workflow.

The plate-based absorbance readout provides 96 assays of material with all reagents supplied to run a full microplate, supporting batched analysis of experimental cohorts. The assay uses a two-antibody capture configuration and is calibrated specifically for rat samples, with protocols validated for tissue homogenates and cell lysates. It is intended for research use only.

Typical workflows involve homogenizing flash-frozen tissue or pelleted cultured cells in a suitable buffer, clarifying the lysate, and running samples alongside a standard curve. Because peripheral TPH1 abundance shifts in disease models and under pharmacological intervention, the kit is often paired with downstream measurement of serotonin (5-HT) or Tph1 transcript analysis to connect protein levels with biosynthetic output.