Rat Tryptophan 5-hydroxylase 1 (Tph1) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is intended for the quantitative measurement of rat Tryptophan 5-hydroxylase 1 (Tph1), an enzyme reported to play a role in serotonin biosynthesis and linked to the regulation of mood, sleep, and appetite. The kit is for research use only and is reactive with rat samples, including serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Quoted performance characteristics include a detection range of 0.625–40 ng/mL and a sensitivity of 0.32 ng/mL. Intra-assay CV is 3.7% and inter-assay CV is 6.9%. Recovery in serum averages 81% (range 80–87%) and in plasma averages 83% (range 80–89%). Linearity has been evaluated across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, 1:8, and 1:16. The target protein has a reported molecular weight of 51,068 Da (UniProt P09810) and is described as a homotetramer belonging to the pterin-dependent aromatic amino acid hydroxylase family.

Detection is colorimetric, and specificity covers natural and recombinant rat Tph1. Synonyms listed in the product information include Tryptophan 5-monooxygenase 1, Tph, TPH1, TPRH, and indoleacetic acid-5-hydroxylase. The indicated research area is cell biology.