Rat Trypsin ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise quantitative measurement of trypsin levels in rat samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Trypsin is an enzyme involved in protein digestion and various physiological processes, and monitoring its levels can support research into digestive disorders, pancreatic diseases, and inflammation.

The kit uses a double-antibody sandwich ELISA format with colorimetric detection read at 450 nm. Components supplied include a dismountable microplate, lyophilized standard, biotin-labeled detection antibody, HRP-Streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, plate sealer, and a technical manual. The kit is stored at 2-8°C for 12 months.

Validated performance data include linearity across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, and recovery ranges with reported average recoveries of 95% in serum, 98% in EDTA plasma, and 93% in heparin plasma.