Rat TRX (Thioredoxin) CLIA Kit (RTES00543)

Thioredoxin (TRX) is a small redox-active protein (~12 kDa) central to cellular thiol-disulfide balance, antioxidant defense, and the regulation of transcription factors including NF-kB. This chemiluminescence-based kit from Assay Genie provides quantitative measurement of TRX in rat samples with an expanded analytical dynamic range relative to conventional absorbance-based ELISA.

Each kit is supplied with a pre-coated microplate, lyophilized standards, detection reagents, and a complete assay protocol. The chemiluminescent detection chemistry produces signal proportional to TRX concentration, supporting applications in oxidative stress research, inflammation studies, and apoptosis pathway investigations. Researchers commonly use this assay to evaluate TRX as a biomarker of redox status in disease models.

The recommended protocol uses a 50 µL sample input per well. Unopened kit components should be refrigerated, while reconstituted standards and conjugate solutions are best kept frozen at -20°C for extended shelf life and stability through multiple freeze-thaw cycles.