Rat Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

Built by Assay Genie for quantitative measurement of rat trefoil factor 3, this in vitro ELISA kit supports research into intestinal mucosal biology, gut barrier function, and gastrointestinal injury models. TFF3 is a small secreted peptide expressed primarily by intestinal goblet cells, where it contributes to epithelial restitution, mucus stabilization, and protection of the mucosa from damage. Researchers studying colitis, NSAID-induced enteropathy, gut barrier dysfunction, and intestinal inflammatory conditions measure TFF3 concentrations in blood-derived samples and tissue homogenates as an indicator of mucosal status and healing responses.

The kit is supplied with a pre-coated microplate, rat-specific standards, detection antibody, and the buffers needed to perform the assay. Optimized for rat reactivity, it is intended for research use only and fits into controlled laboratory workflows for evaluating TFF3 expression across disease models, pharmacological interventions, and time-course experiments. Sample handling should follow typical laboratory procedures, including aliquoting of samples to limit freeze-thaw cycles before running the assay.