Rat Transgelin (TAGLN) ELISA Kit

Transgelin, a 22 kDa actin-binding protein also known as SM22-alpha, serves as a marker of differentiated smooth muscle cells, and the Assay Genie rat-specific ELISA kit enables laboratories to quantify this target across multiple sample matrices in preclinical research workflows. Investigators studying vascular remodeling, fibrotic tissue, pulmonary hypertension, atherosclerosis, and tumor stromal biology rely on circulating transgelin measurements to complement histological readouts in rat disease models.

This microplate-format kit is supplied with pre-coated wells, ready-to-use reagents, calibration standards, and assay buffers needed to establish a standard curve from the first run. Reconstituted components should be kept refrigerated, and unopened kits require cold-chain storage to preserve antibody binding activity through the expiration date printed on the vial labels. The included insert provides recommended dilution ranges for each validated sample matrix, supporting reproducible quantification across treatment groups and replicates.

Typical applications include pairing this ELISA with western blot validation of TAGLN isoforms, qPCR profiling of the corresponding transcript, and immunohistochemistry on matched tissue sections. Whether the study explores smooth muscle phenotypic switching in a carotid injury model, monitors stromal transgelin expression in tumor xenografts, or screens candidate antifibrotic compounds in vivo, the kit provides the analytical sensitivity required for downstream comparison. The pack format contains all consumables necessary to complete a full plate run, making it practical for both focused mechanistic experiments and larger screening campaigns.