Rat TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit

Designed for quantitative measurement of troponin T type 1, slow skeletal (TNNT1) in rat samples, this Sandwich ELISA from Assay Genie uses a double-antibody format and is intended for research applications involving muscle physiology, cardiac health, and muscle-related disorders. The assay supports detection of changes in troponin T levels that may indicate cardiac and skeletal muscle damage, providing a tool for studying muscle function and assessing treatment efficacy.

Key performance characteristics include a sensitivity of 9.375 pg/mL and a working range of 15.625-1000 pg/mL. The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with components stored at 2-8°C for 12 months. Reported linearity for serum (n=5) is 85-104% at 1:2, 92-97% at 1:4, and 89-105% at 1:8; EDTA plasma (n=5) shows 92-97%, 90-101%, and 87-96% at the same dilutions; heparin plasma (n=5) shows 85-97%, 83-94%, and 84-100%. Recovery averages 92% for serum (range 91-101%), 91% for EDTA plasma (range 87-102%), and 96% for heparin plasma (range 90-101%).

The protocol follows a standard sandwich workflow: equilibrate reagents and TMB substrate, load standards and samples for a 90-minute primary incubation at 37°C, add biotin-labeled detection antibody for 60 minutes, add HRP-Streptavidin (SABC) for 30 minutes, develop with TMB substrate in the dark for 10-20 minutes, and read absorbance at 450 nm immediately after adding stop solution.