Rat TNNT1 (Troponin T Type 1, Slow Skeletal) ELISA Kit (RTES01162)

Quantitative measurement of TNNT1 in rat-derived specimens is the purpose of this 96-assay ELISA kit from Assay Genie, supporting investigations into skeletal muscle physiology, fiber-type composition, exercise adaptation, atrophy following denervation, and nemaline myopathy linked to troponin gene mutations. Slow skeletal troponin T is the contractile protein isoform confined to type I slow-twitch fibers, and its circulating or tissue abundance is monitored as a molecular readout of muscle phenotype. The kit supplies paired antibodies, calibration standards, buffers, wash concentrate, and substrate sufficient for a full microplate run. An absorbance-based enzymatic reaction delivers the readout on a standard microplate reader, eliminating the need for radioactivity or fluorescence instrumentation. Recommended specimen types include rat blood-derived fractions, conditioned medium from differentiating rat myotubes, and clarified homogenates from slow-twitch-rich muscles such as soleus or diaphragm. Refrigerate all reagents upon arrival at the temperature indicated on the kit insert and protect the substrate solution from light to preserve reporter activity. The product is intended for research use only and is not validated for clinical diagnostic procedures.