Rat TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) CLIA Kit (RTES00578)

This chemiluminescence immunoassay quantifies soluble TNFRSF1B (also known as TNFR2 or p75 TNF receptor) in rat biological samples. The Assay Genie kit is supplied as a 96-well microplate pre-coated with capture antibody, paired with lyophilized standards and an HRP-conjugated detection reagent that emits signal upon addition of the chemiluminescent substrate.

The working range typically spans tens to thousands of picograms per milliliter, supporting accurate measurement of circulating TNFR2 in serum, plasma, and tissue homogenates. Researchers use this in vitro ELISA to investigate inflammatory signaling, sepsis models, autoimmune disease mechanisms, and receptor-shedding kinetics in rats.

For best results, store unopened components at 2 °C to 8 °C and equilibrate reagents to room temperature before use. Each plate may be run in full or partitioned for smaller studies; the protocol generally requires 50 ul of sample per well with a total incubation of approximately 3.5 hours.