Rat TNF-alpha SuperSet ELISA Kit

The Assay Genie SuperSet format delivers a complete workflow in a single box, with a pre-coated microplate, lyophilized recombinant rat TNF-alpha standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, dilution buffer, substrate solution, and stop solution. Researchers studying inflammatory signaling, septic shock models, or cytokine release phenomena use this kit to quantify TNF-alpha from activated macrophages and to monitor treatment response in arthritis, colitis, or neuroinflammation studies. The assay reliably distinguishes low from high responders in lipopolysaccharide-challenged rat cohorts, with sensitivity below 10 pg/mL and a detection range of 31.25 to 2000 pg/mL. Unopened kits are shipped refrigerated and stored cold on arrival; the reconstituted standard should be aliquoted to limit freeze-thaw cycles. Each component is intended for research use only in in vitro experiments, supporting reproducible, publication-ready data from cell culture supernatants and serum samples.