Rat TNF-alpha PharmaGenie ELISA Kit (SBRS1703)

The Rat TNF-alpha PharmaGenie ELISA Kit from Assay Genie is a quantitative immunoassay for measuring tumor necrosis factor alpha in rat samples. TNF-alpha is a key pro-inflammatory cytokine involved in systemic inflammation, immune regulation, and apoptotic cell signaling, making accurate quantification essential in studies of rheumatoid arthritis models, septic shock, neuroinflammation, and metabolic disease.

The assay uses a biotin-streptavidin amplification format and includes a pre-coated microplate, lyophilized recombinant rat TNF-alpha standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and assay diluent.

Reconstituted standards and diluted detection antibody should be stored at -20 °C; protect reagents from light during incubation steps and avoid repeated freeze-thaw cycles for working dilutions. Researchers apply this kit to investigate cytokine release from stimulated macrophages, evaluate anti-inflammatory compound efficacy in rodent models, and profile inflammatory cascades in tissue lysates from experimental disease models.