Rat TNF-alpha ELISpot Kit

The Assay Genie Rat TNF-alpha ELISpot Kit enables single-cell resolution detection of TNF-alpha secretion in rat samples, supporting immunology, vaccine, and inflammation research. Each kit provides a pre-coated microplate with rat-specific capture antibody, biotinylated detection antibody, streptavidin-enzyme conjugate, and substrate for colorimetric spot development. The assay accommodates cell culture supernatants and serum, fitting into cell culture and in vitro workflows. Researchers quantify TNF-alpha-producing cells by counting spot-forming units with an automated reader, reaching single-digit pg/mL sensitivity per spot. Reagents should be stored at 4°C and equilibrated to room temperature before use; the kit is intended for research use only.