Rat TLR9 (Toll-Like Receptor 9) ELISA Kit (RTES00830)

The Assay Genie TLR9 immunoassay kit is configured for quantitative measurement of Toll-Like Receptor 9 in rat-derived research specimens, supporting immunology, inflammation, and innate immunity studies. Each kit contains reagents sufficient for 96 individual determinations run on a standard microplate reader.

TLR9 is an intracellular pattern-recognition receptor localized to endosomal compartments, where it senses unmethylated CpG motifs characteristic of bacterial and viral DNA. Researchers working with rat models of autoimmune disease, vaccine response, sepsis, or mucosal inflammation use this receptor as a biomarker for innate immune activation. The kit accommodates a range of rat-derived sample types commonly used in rodent preclinical workflows, including serum fractions, plasma preparations, and tissue homogenates.

The 96-assay microplate format uses a ready-to-use capture plate pre-coated with anti-TLR9 capture antibody, along with biotinylated detection reagent, streptavidin-HRP conjugate, TMB substrate, and a calibrated protein standard supplied as separate components. Reagent storage conditions are detailed in the product insert: unopened kits are kept refrigerated until first use, while reconstituted standard aliquots are stored frozen in single-use vials to limit freeze-thaw cycles.

Assay Genie provides protocol documentation and technical support for laboratories running vaccine development, host-pathogen interaction, and biomarker discovery programs in rat model systems.