Rat Tissue factor/Coagulation Factor III ELISA Kit

Designed for the precise measurement of tissue factor levels in rat-derived samples, this Sandwich ELISA from Assay Genie uses a double-antibody configuration to detect Tissue factor (Coagulation Factor III). The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a working range of 31.25–2000 pg/mL and a sensitivity of 18.75 pg/mL. Kit components are stored at 2–8°C for up to 12 months.

Validation data show strong linearity across 1:2, 1:4, and 1:8 dilutions: serum 90–104%, 89–102%, 90–103%; EDTA plasma 83–95%, 84–96%, 84–97%; heparin plasma 82–93%, 85–94%, 80–89%. Recovery averaged 94% for serum (range 87–103%), 95% for EDTA plasma (range 89–100%), and 91% for heparin plasma (range 88–98%). Tissue factor—also referred to as TF, F3, FIII, CD142, TFA, Thromboplastin, or Coagulation Factor III—is a central initiator of the coagulation cascade, and its dysregulation has been linked to thrombosis, inflammation, and cancer progression, making it a useful biomarker for investigating these conditions.