Rat TIMP2/Metalloproteinase inhibitor 2 ELISA Kit

Assay Genie offers a Sandwich ELISA, Double Antibody-based kit designed for the quantitative measurement of TIMP2 (Metalloproteinase Inhibitor 2) in rat serum, plasma, and cell culture supernatants. The assay targets Rat TIMP-2, also referred to as TIMP-2, Metalloproteinase inhibitor 2, and Tissue inhibitor of metalloproteinases 2, supporting research into extracellular matrix degradation, tissue remodeling, and related disease pathways.

Key performance specifications include a sensitivity of 0.188 ng/ml, an assay range of 0.313–20 ng/ml, intra-assay CV <8%, and inter-assay CV <10%. Recovery testing in serum (n=5) yielded 86–103% (average 97%), in EDTA plasma (n=5) 86–105% (average 93%), and in UFH plasma (n=5) 91–102% (average 97%). Linearity was confirmed across serial dilutions of spiked serum, EDTA plasma, and UFH plasma samples. The kit is stored at 4°C for 6 months and is intended For Research Use Only.

At the protein level, TIMP2 complexes with metalloproteinases (including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19) and irreversibly inactivates them by binding to their catalytic zinc cofactor. The protein has a reported molecular weight of 24,356 Da and corresponds to UniProt entry P30121. Dysregulation of TIMP2 has been linked to cancer, inflammation, and cardiovascular disorders, underscoring its relevance as a research target and potential diagnostic marker.