Rat TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) CLIA Kit (RTES00557)

Expanding on the core specifications, this chemiluminescent immunoassay from Assay Genie provides a quantitative working range typically spanning low pg/mL to mid-ng/mL concentrations, with a detection limit in the sub-picogram range that supports precise measurement of endogenous TIMP-2 in dilute samples. The assay procedure follows a standard sandwich format: diluted standards and unknowns are incubated in pre-coated microwells, followed by a biotinylated detection antibody and streptavidin-HRP conjugate, with signal generated by a luminol-based substrate and read on a chemiluminescence plate reader.

Typical intra-assay precision is below 8% CV and inter-assay precision below 12% CV, with reproducibility validated across multiple rat strains including Sprague-Dawley and Wistar. Recommended sample volumes are 50-100 µL per well for serum and plasma, and tissue homogenates may be analyzed after appropriate dilution in assay buffer. The full protocol can be completed within approximately 3-4 hours, and the kit is compatible with EDTA, heparin, and citrate plasma collection tubes.

Beyond the applications already noted, TIMP-2 quantification in rat models is frequently used in renal injury studies (where TIMP-2 combined with IGFBP7 has emerged as an early biomarker of tubular stress), hepatic fibrosis models triggered by CCl4 or bile duct ligation, myocardial remodeling after ischemic injury, and tumor xenograft progression studies. The 96-well strip format allows processing of partial batches; broken strips can be returned to the foil pouch with desiccant and stored refrigerated at 2-8 °C to preserve antibody activity across multi-day workflows.