Rat TIMP-2 PharmaGenie ELISA Kit (SBRS1702)

The Rat TIMP-2 protein (Tissue Inhibitor of Metalloproteinases 2) is a key regulator of extracellular matrix turnover, and quantifying its concentration is valuable in studies of fibrosis, tumor invasion, cardiac remodeling, and wound healing research. This ELISA from Assay Genie uses pre-coated capture antibodies specific to rat TIMP-2, enabling researchers to measure endogenous protein levels across multiple sample matrices including cell culture supernatant, plasma, and serum specimens.

The microplate-format kit includes a pre-bound anti-TIMP-2 plate, lyophilized recombinant standards, a biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate solution, and stop solution. Researchers add standards and samples to wells, incubate, wash, and add the detection reagent to form the antibody-antigen-antibody complex.

TIMP-2 quantification supports investigations into MMP/TIMP balance, which influences collagen deposition, basement membrane integrity, and cellular signaling pathways involving ERK and Akt kinases. The kit is intended for in vitro research use only and supports biomarker validation studies.