Rat Tight Junction Protein 1 (TJP1) ELISA Kit

Targeting the tight junction scaffold protein ZO-1, this rat-reactive ELISA kit from Assay Genie provides quantitative measurement in tissue homogenates and cell lysates. TJP1, also known as Zonula Occludens-1, anchors the claudin and occludin strands of tight junctions to the actin cytoskeleton, making it a key marker for epithelial and endothelial barrier integrity in neuroscience, gastroenterology, nephrology, and pulmonary research.

Researchers studying blood-brain barrier disruption, intestinal permeability, renal tubular function, and lung epithelial damage routinely track ZO-1 expression to evaluate barrier compromise in disease models and to test the effect of pharmacological interventions. The 96-assay format supports multiple experimental conditions and replicates per plate.

Each kit includes pre-coated microplate wells, biotinylated detection antibody, standards, and the reagents needed to generate a standard curve. The colorimetric end-point is read on a standard microplate reader, allowing labs without specialized luminometer or fluorometer equipment to incorporate tight junction analysis into existing workflows.

For research use only, the kit supports investigations into mechanisms of inflammation-driven barrier dysfunction, pathogen translocation, drug-induced epithelial injury, and tight junction remodeling during development or carcinogenesis. Store reagents according to the package insert to maintain assay performance across runs.