Rat TGF- alpha (Transforming Growth Factor Alpha) ELISA Kit (RTES00846)

Beyond simple quantification, this Assay Genie kit supports research into TGF-alpha signaling in rat models. TGF-alpha is a 50 amino acid EGF-family ligand that activates the epidermal growth factor receptor (EGFR), driving studies in epithelial proliferation, gastrointestinal mucosal healing, hepatic regeneration, and tumor biology. The reported detection range is 15.6 to 1000 pg/mL with a sensitivity around 4.7 pg/mL, using a 100 uL sample loading volume per well. All reagents are supplied ready-to-use except for the lyophilized recombinant rat TGF-alpha standard curve, which is reconstituted with the provided diluent and may be aliquoted and frozen for repeated freeze-thaw cycles. Unopened kits should be stored refrigerated at 2-8°C and protected from light, with a shelf life of approximately 6 months under these conditions. The complete workflow finishes in about 3 hours at room temperature. Components include a pre-coated microplate strip, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, stop solution, and standard diluent, supporting reproducible cytokine quantitation across cancer, wound healing, and EGF-family crosstalk investigations.