Rat TFPI/Tissue factor pathway inhibitor ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit uses a double antibody format for the quantitative measurement of Tissue Factor Pathway Inhibitor (TFPI) in rat samples. The assay is colorimetric and detects the target in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, with reported sensitivity of 37.5 pg/mL and a working range of 62.5-4000 pg/mL.

TFPI is a key inhibitor of the coagulation pathway, helping to regulate blood clot formation and maintain vascular health. The kit includes linearty validation across serum, EDTA plasma and heparin plasma at 1:2, 1:4 and 1:8 dilutions, with serum recovery averaging 92%, EDTA plasma averaging 93% and heparin plasma averaging 99%. Reagents are stored at 2-8°C for 12 months, and the workflow involves a 90 minute primary incubation at 37°C, biotin-labelled detection antibody binding, HRP-Streptavidin (SABC) binding, TMB substrate colour development and absorbance reading at 450 nm.