Rat TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit (RTES00857)

Beyond basic quantification, this Assay Genie rat TFF3 ELISA kit supports research into gastrointestinal mucosal biology, where Trefoil Factor 3 serves as a biomarker for epithelial repair, goblet cell integrity, and barrier function in studies of inflammatory bowel disease, gastric ulceration, and intestinal injury models.

The assay is supplied as a complete microplate kit with pre-coated capture antibody, lyophilized recombinant rat TFF3 standards, biotinylated detection reagent, and streptavidin-HRP conjugate. A 50 uL sample volume is added to each well, and rat-specific antibodies are used to minimize cross-reactivity with homologous trefoil peptides from other species.

Reagents should be kept refrigerated between uses, with the standard stock aliquoted and frozen at -20 degrees C to limit freeze-thaw loss of binding activity. Reconstituted working standards remain stable through a limited number of freeze-thaw cycles when handled promptly.