Rat TFF3/Trefoil Factor 3 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise quantitative measurement of Trefoil Factor 3 (TFF3) in rat samples, supporting research into gastrointestinal health and disease pathology. The kit uses a double-antibody sandwich format with colorimetric detection and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

TFF3 is an important protein known for its involvement in promoting mucosal healing and protecting the gastrointestinal tract, with relevance to conditions such as inflammatory bowel disease, gastric ulcers, and colorectal cancer. The assay offers a sensitivity of 75 pg/mL and a working range of 125-8000 pg/mL, with absorbance read at 450 nm after TMB substrate development. Reported linearity ranges from 80-104% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, with recovery averages of 98% (serum), 93% (EDTA plasma), and 94% (heparin plasma). Components should be stored at 2-8°C for up to 12 months.