Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit (RTES00792)

Assay Genie offers a colorimetric sandwich ELISA for the quantitative measurement of terminal complement complex C5b-9 in rat samples. The kit is reactive with rat and is intended for serum, plasma, cell culture supernatants and other biological fluids, with a sample volume of 100 μL. MAC, membrane attack complex, is listed as a synonym for the analyte.

Stated performance specifications are a sensitivity of 0.1 ng/mL, a detection range of 0.16-10 ng/mL and recovery of 80%-120%. The assay time is listed as 3 h 30 min and storage as 2-8°C. Linearity is reported for serum, EDTA plasma and cell culture media at dilutions of 1:2, 1:4, 1:8 and 1:16, and precision is given as intra-assay CV values of 4.15%, 5.85% and 6.9% with inter-assay CV values of 8.89%, 6.31% and 6.38%. The kit recognizes rat sC5b-9, and no significant cross-reactivity or interference between rat sC5b-9 and analogues was observed.

Listed components include a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, sample and detection antibody diluents, HRP conjugate diluent, wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. The manufacturer lists the research areas as cell biology, metabolism, immunology and signal transduction.