Rat TBG/SERPINA7 ELISA Kit

Assay Genie's Rat TBG/SERPINA7 ELISA Kit is a sandwich ELISA designed for the precise measurement of Thyroxine-Binding Globulin (TBG), also known as Serpina7, in rat samples. The kit uses a double-antibody sandwich format with colorimetric detection and is intended for research into thyroid function, since TBG plays a crucial role in the transport of thyroid hormones in the bloodstream and changes in its levels have been associated with thyroid disorders and hormonal imbalances.

According to the manufacturer, the assay has a sensitivity of 0.375 ng/mL and a working range of 0.625–40 ng/mL. It accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and the kit is stored at 2–8°C for 12 months. Reported linearity spans roughly 83–105% across serum, EDTA plasma, and heparin plasma at 1:2 to 1:8 dilutions, with average spike-and-recovery of 96% for serum, 91% for EDTA plasma, and 96% for heparin plasma.

The protocol involves equilibration of reagents, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding for 60 minutes, HRP-streptavidin (SABC) binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and a stop reaction read at 450 nm. Listed target synonyms include Serpina7, SERPINA7, Serpin A7, TBG, Thyroxine-binding globulin, and T4-binding globulin, with UniProt ID P35577.