Rat TARC (Thymus Activation Regulated Chemokine) ELISA Kit (RTES00810)

Assay Genie positions this chemokine panel for researchers investigating the CCR4-CCL17 axis, where CCL17 (gene symbol Ccl17, also known as small-inducible cytokine A17 / SCYA17 and ABCD-2) functions as the principal chemoattractant for CCR4-expressing Th2 lymphocytes and cutaneous lymphocyte-associated antigen-positive (CLA+) T cells. In rat disease models, circulating TARC concentrations spike during the late-phase response of ovalbumin-sensitized Brown Norway airways, track with eosinophil counts in house dust mite (HDM) challenge protocols, and are elevated in bleomycin-induced pulmonary fibrosis lung homogenates. Dermatologic applications extend to imiquimod- and 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis-like lesions, where lesional biopsies show TARC induction paralleling epidermal thickening. For sample handling, serum should be clotted for 2 hours at room temperature, centrifuged at 1000 x g for 20 minutes, aliquoted, and stored at -20 °C or below; avoid more than two freeze-thaw cycles and read the plate within 10 minutes of adding stop solution to minimize absorbance drift.