Rat Syndecan-4/SDC4 ELISA Kit

Quantify rat Syndecan-4 (SDC4) with this colorimetric microplate immunoassay from Assay Genie. Syndecan-4 is a single-pass transmembrane heparan sulfate proteoglycan that acts as a co-receptor at focal adhesions, integrating extracellular matrix cues with intracellular cytoskeletal signaling. Dysregulated SDC4 expression has been documented in models of cardiac fibrosis, osteoarthritis, wound repair, and vascular injury, where the proteoglycan contributes to mechanotransduction and inflammatory cytokine responses.

Each Assay Genie kit ships as a complete package containing pre-coated microplate wells, lyophilized standards, biotinylated detection reagent, streptavidin-enzyme conjugate, wash concentrate, and substrate solution. Researchers aliquot rat serum or plasma specimens into the wells alongside a dilution series of standards, then follow the step-wise incubation protocol to generate a quantitative readout. The absorbance measured at the assay endpoint correlates with SDC4 concentration, enabling comparison between treatment groups in pharmacological or genetic studies.

Unopened kits should be refrigerated and stored away from light to preserve reagent activity; reconstituted standards are best held frozen in single-use aliquots to limit repeated freeze-thaw cycles. Running the immunoassay on freshly collected or properly banked rat specimens helps preserve the epitopes recognized by the capture antibody. The microplate layout supports parallel processing of up to 96 determinations per run, accommodating medium-throughput screening in academic and translational research laboratories.