Rat Syndecan 4 (SDC4) ELISA Kit

A quantitative immunoassay from Assay Genie, the Rat Syndecan 4 (SDC4) ELISA Kit enables measurement of SDC4, a transmembrane heparan sulfate proteoglycan that participates in focal adhesion assembly, integrin signaling, and protein kinase C-alpha activation at the cell surface. The kit is calibrated for rat samples and supports research on extracellular matrix biology, wound healing, inflammation, fibrosis, and tumor microenvironments.

Each kit contains a pre-coated microplate, biotinylated detection antibody, reference standard, and chromogenic substrate for absorbance-based readout on a standard plate reader. Standard incubation steps are carried out at 37 °C, and researchers should store reagents refrigerated between uses to preserve antibody activity. A standard curve generated from serial dilutions of the supplied reference allows interpolation of unknown sample concentrations.

The assay accepts liquid samples derived from rat blood fractions, solubilized tissue preparations, and cultured cell lysates, supporting both systemic and cell-based study designs. SDC4 shedding from the cell surface is a regulated process, and circulating levels are studied as indicators of tissue remodeling and disease activity in cardiac, renal, and pulmonary models.