Rat Synaptophysin ELISA Kit

From Assay Genie, this Rat Synaptophysin ELISA Kit is a sandwich ELISA, double antibody assay designed for the precise measurement of synaptophysin levels in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Synaptophysin is a key synaptic vesicle protein involved in neurotransmitter release, and its dysregulation has been linked to neurological disorders such as Alzheimer's disease, Parkinson's disease, and schizophrenia, making it a valuable biomarker for research into these conditions.

The kit has a reported sensitivity of 0.375 ng/mL and a range of 0.625-40 ng/mL. Detection is colorimetric, with absorbance measured at 450 nm. Storage is at 2-8°C for 12 months. Reported linearity in serum (n=5) ranges from 85-103%, in EDTA plasma (n=5) from 83-100%, and in heparin plasma (n=5) from 81-99%. Reported average recovery is 95% for serum, 94% for EDTA plasma, and 95% for heparin plasma.

The assay procedure involves equilibrating reagents to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and a stop reaction before reading absorbance at 450 nm.