Rat Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit

Succinate dehydrogenase subunit B (SDHB) anchors the iron-sulfur clusters of mitochondrial Complex II, the succinate-ubiquinone oxidoreductase that couples the TCA cycle to the electron transport chain. Quantifying SDHB protein levels in rat samples gives researchers a window into mitochondrial respiratory integrity, metabolic reprogramming, and hypoxic signaling relevant to neuroscience, cardiology, and tumor biology.

Assay Genie supplies this rat-reactive ELISA kit in a 96-assay configuration validated for tissue homogenates and cell lysate preparations from rat sources. Investigators working on paraganglioma-predisposition models, ischemic injury, or inherited mitochondrial myopathies use this assay to compare SDHB expression across treatment cohorts, genetic backgrounds, or subcellular fractions.

The kit includes pre-coated plate wells, detection reagents, and a recombinant rat SDHB standard curve sufficient for quantitative interpolation of unknown samples. Storage at refrigerated temperatures preserves antibody and standard stability. An absorbance-based readout integrates with standard microplate reader workflows without specialized instrumentation, supporting routine bench use.

Typical downstream workflows pair the assay with western blot confirmation, qPCR of SDHB transcript, and Complex II enzymatic activity measurements, making this kit a practical anchor for studies where SDHB loss-of-function drives the experimental question, including HIF pathway activation, ROS production, and TCA cycle flux analysis. Intended for research use only.