Rat sTNF RII/TNFRSF1B ELISA Kit

Designed for rat samples, this ELISA kit from Assay Genie enables the precise measurement of TNFRSF1B (also known as Tumor Necrosis Factor Receptor Superfamily Member 1B, or sTNF RII), a receptor involved in the regulation of inflammatory responses and cell death pathways. It is intended for research into inflammation, immune responses, and cell signalling pathways in rat models, where accurate detection of TNFRSF1B levels can provide insights into disease mechanisms and potential therapeutic targets.

The assay is a sandwich ELISA using a double-antibody format with colorimetric detection. Sensitivity is quoted at 9.375 pg/mL, with a working range of 15.625-1000 pg/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for 12 months.

Reported linearity (n=5) across 1:2, 1:4 and 1:8 dilutions is 89-95% / 88-100% / 80-99% for serum, 96-104% / 86-101% / 82-97% for EDTA plasma, and 89-103% / 84-99% / 82-97% for heparin plasma. Recovery (n=5) is 86-105% (average 95%) for serum, 87-104% (average 94%) for EDTA plasma, and 88-105% (average 97%) for heparin plasma. The assay procedure involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody for 60 minutes, HRP-Streptavidin (SABC) for 30 minutes, TMB substrate in the dark for 10-20 minutes, and absorbance reading at 450 nm after adding stop solution.