Rat SPARC/Osteonectin ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit uses a double-antibody format with colorimetric detection to quantify SPARC (also known as osteonectin) in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. The assay is specifically designed for the precise measurement of SPARC levels, offering sensitivity and specificity suitable for a range of research purposes.

The kit delivers a sensitivity of 18.75 pg/mL with a working range of 31.25-2000 pg/mL, and components are stable when stored at 2-8°C for 12 months. Linearity has been validated across serum, EDTA plasma, and heparin plasma at dilution ratios of 1:2, 1:4, and 1:8; for example, serum linearity spans 88-105% across these dilutions. Recovery has also been documented, with serum averaging 95% (range 88-104%), EDTA plasma averaging 92% (range 90-94%), and heparin plasma averaging 95% (range 86-105%).

SPARC, also referred to as ON, BM-40, or Secreted Protein Acidic and Rich in Cysteine, is a critical protein involved in regulating cell-matrix interactions and tissue remodeling, and plays key roles in wound healing, bone formation, and tumorigenesis. By measuring SPARC levels, researchers can gain insights into pathological conditions affecting these processes and potentially identify therapeutic targets, making this kit a reliable tool for studying SPARC in various biological processes and disease states.