Rat SP (Substance P) ELISA Kit (RTFI01443)

The Assay Genie Rat Substance P ELISA provides researchers with a quantitative tool for measuring SP neuropeptide levels in experimental rat samples. Substance P is an 11-residue tachykinin neurotransmitter implicated in nociception, neurogenic inflammation, and stress response pathways, making this assay relevant for neuroscience and pharmacology laboratories. The kit includes a pre-coated microplate, lyophilized standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, and substrate solution in individually sealed pouches to preserve reagent stability. Refrigerate all unopened components and protect the HRP conjugate from light; aliquot the standard after reconstitution to limit freeze-thaw exposure. The procedure supports 50 uL sample volumes run in duplicate and completes within a standard immunoassay workflow. This ELISA is intended for in vitro research use only and is not approved for diagnostic applications.