Rat SOCS1 ELISA Kit

Designed by Assay Genie, this sandwich ELISA, double-antibody kit enables quantitative measurement of Suppressor of Cytokine Signaling 1 (SOCS1) in rat samples. The kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, supporting studies of immune regulation and inflammation in contexts such as autoimmune disease, infectious disease, and cancer.

Performance specifications include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Recovery testing across serum, EDTA plasma and heparin plasma showed recoveries from 85–103%, averaging 93–94%, while dilution linearity at 1:2, 1:4 and 1:8 spanned 82–103% across the same matrices. The workflow uses a biotinylated detection antibody with HRP-Streptavidin (SABC) and TMB substrate, and absorbance is read at 450 nm after a 10–20 minute colour development step. Unopened reagents are held at 2–8°C for 12 months.