Rat Smad4/MADH4 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the accurate detection of SMAD4 (also known as MADH4) levels in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The colorimetric, double-antibody sandwich assay has a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL.

SMAD4 is a critical protein in the TGF-β signaling pathway that regulates gene expression and cellular responses to growth factors, with dysregulation linked to cancer, fibrosis, and autoimmune disorders. The kit is categorized under the Epigenetics and Nuclear Signaling research area and targets UniProt O70437 (KEGG K04501; GeneID 50554). Listed synonyms include Smad4, MADH4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, and Hsmad4.

The kit is stored at 2-8°C for 12 months. The assay procedure comprises primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10–20 minutes, and absorbance reading at 450 nm after stop solution addition. Reported linearity across serum, EDTA plasma, and heparin plasma (n = 5) at 1:2, 1:4, and 1:8 dilutions ranges from 82-103%. Recovery averages are 95% for serum, 94% for EDTA plasma, and 98% for heparin plasma.