Rat SLIT2 (Slit Homolog 2) ELISA Kit (RTES00739)

This colorimetric ELISA from Assay Genie quantifies Slit Homolog 2 (SLIT2), a secreted extracellular matrix protein best known for its role as an axon guidance cue through Roundabout (Robo) receptor binding. In rat-derived samples, the assay supports research into neural development, retinal ganglion cell pathfinding, leukocyte chemotaxis, vascular patterning, and tumor cell migration, since SLIT2 acts as a chemorepellent in many of these contexts.

The kit contains pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant rat SLIT2 standards, and substrate solution. It is supplied as a pack of 96 assays for use with standard strip plate frames. Researchers run it with serum, culture supernatant, or homogenized tissue lysates in in vitro studies. The procedure follows a standard capture-and-detect antibody workflow with absorbance reading in the visible spectrum, compatible with common plate readers in a basic lab.

SLIT2 measurement is useful when studying conditions where the SLIT-Robo axis is dysregulated, including diabetic nephropathy models, cancer angiogenesis work, and neural regeneration studies in rodent systems.