Rat Sirtuin 1 (SIRT1) ELISA Kit

SIRT1, an NAD-dependent class III deacetylase, can be quantified from rat tissues and cell lysates with this immunoassay from Assay Genie. The target protein regulates chromatin remodeling, metabolic gene expression, and cellular stress responses in rat model systems, and researchers working on caloric restriction, insulin signaling, neurodegeneration, and longevity commonly use SIRT1 protein levels as a measurable biomarker. The format is supplied as a 96-assay kit that is read on a standard microplate reader.

Reagents include a pre-coated plate, calibrated reference standards, detection antibody, substrate, and buffers suited to tissue homogenates and cell lysate preparation. A typical workflow involves diluting samples into assay buffer, generating the standard curve, and interpolating unknowns from absorbance readings. SIRT1 expression fluctuates with feeding state and circadian timing, so consistent collection and handling protocols help keep longitudinal comparisons reliable across treatment cohorts.

Strip wells and reagents should be held under refrigerated storage between sessions to preserve antibody binding activity across repeated experiments. The product is intended for research use only and is not validated for diagnostic procedures.