Rat Sialidase 2, Cytosolic (SIAL2) ELISA Kit

The Rat Sialidase 2, Cytosolic (SIAL2) ELISA Kit from Assay Genie enables quantitative measurement of NEU2, a cytosolic hydrolytic enzyme that cleaves terminal sialic acid residues from glycoproteins and glycolipids. Researchers studying carbohydrate metabolism, ganglioside catabolism, and lysosomal storage disorders use this in vitro assay to explore NEU2 expression in skeletal muscle, brain, and peripheral tissues.

Unopened kits should be stored at 4 degrees C; reconstituted reagents and standard dilutions can be frozen in aliquots to minimize repeated freeze-thaw cycles. Each microplate-format kit ships with a pre-coated strip plate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and a serial dilution standard curve. A 50 uL sample volume per well is loaded alongside 50 uL of diluted standard or sample diluent.

This kit shows species-specific reactivity with rat NEU2 and supports research use only applications, including investigations into cancer glycosylation, diabetes, and neuromuscular disease models. For intracellular target analysis, tissue homogenates from rat liver, muscle, or brain are commonly prepared in cold PBS with protease inhibitors before loading.